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rabbit anti-pdlim3 antibody (Servicebio Inc)

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Servicebio Inc rabbit anti-pdlim3 antibody
The statistical metrics for key differentially expressed genes (DEGs).

Rabbit Anti Pdlim3 Antibody, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-pdlim3 antibody/product/Servicebio Inc
Average 90 stars, based on 1 article reviews

rabbit anti-pdlim3 antibody - by Bioz Stars, 2026-05

90/100 stars

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1) Product Images from "Bioinformatical analysis identifies PDLIM3 as a potential biomarker associated with immune infiltration in patients with endometriosis"

Article Title: Bioinformatical analysis identifies PDLIM3 as a potential biomarker associated with immune infiltration in patients with endometriosis

Journal: PeerJ

doi: 10.7717/peerj.13218

Figure Legend Snippet: The statistical metrics for key differentially expressed genes (DEGs).

Techniques Used:

Figure Legend Snippet: The enriched GO terms of differentially expressed genes.

Techniques Used: Activation Assay, Clinical Proteomics, Membrane, Binding Assay, Activity Assay

(A) A PPI network of DEGs. (B) A plot of biomarkers selection by LASSO regression algorithm. (C) The receiver operating characteristic (ROC) curve of FMO1, PDLIM3, FAM129A, CLDN11, C3, TMPRSS4 and DEFB1 in the metadata cohort. (D) ROC curve of FMO1, PDLIM3, FAM129A, CLDN11, C3, TMPRSS4 and DEFB1 in the GSE120103 dataset.

Figure Legend Snippet: (A) A PPI network of DEGs. (B) A plot of biomarkers selection by LASSO regression algorithm. (C) The receiver operating characteristic (ROC) curve of FMO1, PDLIM3, FAM129A, CLDN11, C3, TMPRSS4 and DEFB1 in the metadata cohort. (D) ROC curve of FMO1, PDLIM3, FAM129A, CLDN11, C3, TMPRSS4 and DEFB1 in the GSE120103 dataset.

Techniques Used: Selection

(A) The expression level of FMO1, PDLIM3, FAM129A, CLDN11, C3, TMPRSS4 and DEFB1 in the GSE120103 dataset. (B) The mRNA expression level of PDLIM3 in endometriosis and control samples. (C) The protein expression level of PDLIM3 in endometriosis and control samples. Mean ± SD, *** P < 0.001.

Figure Legend Snippet: (A) The expression level of FMO1, PDLIM3, FAM129A, CLDN11, C3, TMPRSS4 and DEFB1 in the GSE120103 dataset. (B) The mRNA expression level of PDLIM3 in endometriosis and control samples. (C) The protein expression level of PDLIM3 in endometriosis and control samples. Mean ± SD, *** P < 0.001.

Techniques Used: Expressing, Control

(A) Correlation between PDLIM3 and single type of immune cell. (B) A comprehensive correlation analysis of PDLIM3 and infiltrating immune cells in endometriosis.

Figure Legend Snippet: (A) Correlation between PDLIM3 and single type of immune cell. (B) A comprehensive correlation analysis of PDLIM3 and infiltrating immune cells in endometriosis.

Techniques Used:

Image Search Results

The images of H.E. staining and immunohistochemistry staining for prenatal rat dental germs. H.E. staining demonstrated that the rat dental germs entered the early cap stage at E14.5 d, the cap stage and early bell stage at E16.5 d, and the bell stage at E18.5 d. The separation between the pre-odontoblast and pre-ameloblast layers occurred in all E18.5 d species. Immunohistochemistry staining revealed that Clock , Per1 , and Col1 were detected in the epithelial-mesenchymal interaction area, dental follicle, and dental papilla at E14.5 d, and became stronger at E16.5 d when p75NTR , Bmal1 , and ALP were detected. All the factors were expressed at E18.5 d, but Cry1 showed the weakest expression. All experiments were repeated three times independently. o p : oral epithelium; dp: dental papilla; iee: inner enamel epithelium; oee: outer enamel epithelium; sr: stellate reticulum. The scale bar represents 50 μm, respectively.

Journal: Frontiers in Physiology

Article Title: A potential role of p75NTR in the regulation of circadian rhythm and incremental growth lines during tooth development

doi: 10.3389/fphys.2022.981311

Figure Lengend Snippet: The images of H.E. staining and immunohistochemistry staining for prenatal rat dental germs. H.E. staining demonstrated that the rat dental germs entered the early cap stage at E14.5 d, the cap stage and early bell stage at E16.5 d, and the bell stage at E18.5 d. The separation between the pre-odontoblast and pre-ameloblast layers occurred in all E18.5 d species. Immunohistochemistry staining revealed that Clock , Per1 , and Col1 were detected in the epithelial-mesenchymal interaction area, dental follicle, and dental papilla at E14.5 d, and became stronger at E16.5 d when p75NTR , Bmal1 , and ALP were detected. All the factors were expressed at E18.5 d, but Cry1 showed the weakest expression. All experiments were repeated three times independently. o p : oral epithelium; dp: dental papilla; iee: inner enamel epithelium; oee: outer enamel epithelium; sr: stellate reticulum. The scale bar represents 50 μm, respectively.

Article Snippet: The primary antibodies were used in this study are as follows: rabbit anti-rat p75NTR (1:1,500; Abcam, Cambridge, MA, United States, ab245134, monoclonal), rabbit anti-rat BMAL1 (1:1,000; Abcam, Cambridge, MA, United States, ab230822, monoclonal), rabbit anti-rat CLOCK (1:1,000; Abcam, Cambridge, MA, United States, ab3517, polyclonal), rabbit anti-rat PER1 (1:500; Bioss, Beijing, China,bs-2350R, polyclonal), rabbit anti-rat CRY1 (1:500; Bioss, Beijing, China bs-11441R, polyclonal), rabbit anti-rat ALP (1:500; Bioss, Beijing, China, bs-2928R, polyclonal), rabbit anti-rat COL1 (1:500; Bioss, Beijing, China, bs-0578R, polyclonal).

Techniques: Staining, Immunohistochemistry, Expressing

Mice oligonucleotide primers used in this study.

Journal: Frontiers in Physiology

Article Title: A potential role of p75NTR in the regulation of circadian rhythm and incremental growth lines during tooth development

doi: 10.3389/fphys.2022.981311

Figure Lengend Snippet: Mice oligonucleotide primers used in this study.

Techniques:

The in vivo observation of circadian rhythm dynamics in PN7 d dental germs of wild-type mice. p75NTR mRNA showed a significant difference between D.D. and L.D. conditions ( p < 0.05), but the change tendencies at two sampling times were opposite: p75NTR mRNA expression in the L.D. condition was significantly higher at 7:30 a.m. and significantly lower at 7:30 p.m. in the D.D. condition ( p < 0.05). The expression pattern of Mage-D1 was similar to that of p75NTR. Most factors ( Bmal1 , Clock , Per1 , Per2 , Runx2 , ALP , Col1 , and Dlx1 ) showed the same change tendency at the two sampling times: mRNA expression was significantly higher in the D.D. condition than that in the L.D. condition ( p < 0.05). The change amplitudes of Clock , Per1 , Per2 , Runx2 , and Dlx1 were significantly larger at the sampling time of 7:30 PM. Contrary to p75NTR , Msx1 mRNA expression in the L.D. condition was significantly lower at 7:30 a.m. and significantly higher at 7:30 p.m. compared with the D.D. condition ( p < 0.05). Dmp1 and Dspp mRNA expression in the D.D. condition was significantly lower than that in L.D. condition at both sampling times ( p < 0.05). All experiments were repeated at least three times independently. Data are expressed as mean ± S.D. L. D. Condition group, n = 11; D. D. Condition group, n = 5.

Journal: Frontiers in Physiology

Article Title: A potential role of p75NTR in the regulation of circadian rhythm and incremental growth lines during tooth development

doi: 10.3389/fphys.2022.981311

Figure Lengend Snippet: The in vivo observation of circadian rhythm dynamics in PN7 d dental germs of wild-type mice. p75NTR mRNA showed a significant difference between D.D. and L.D. conditions ( p < 0.05), but the change tendencies at two sampling times were opposite: p75NTR mRNA expression in the L.D. condition was significantly higher at 7:30 a.m. and significantly lower at 7:30 p.m. in the D.D. condition ( p < 0.05). The expression pattern of Mage-D1 was similar to that of p75NTR. Most factors ( Bmal1 , Clock , Per1 , Per2 , Runx2 , ALP , Col1 , and Dlx1 ) showed the same change tendency at the two sampling times: mRNA expression was significantly higher in the D.D. condition than that in the L.D. condition ( p < 0.05). The change amplitudes of Clock , Per1 , Per2 , Runx2 , and Dlx1 were significantly larger at the sampling time of 7:30 PM. Contrary to p75NTR , Msx1 mRNA expression in the L.D. condition was significantly lower at 7:30 a.m. and significantly higher at 7:30 p.m. compared with the D.D. condition ( p < 0.05). Dmp1 and Dspp mRNA expression in the D.D. condition was significantly lower than that in L.D. condition at both sampling times ( p < 0.05). All experiments were repeated at least three times independently. Data are expressed as mean ± S.D. L. D. Condition group, n = 11; D. D. Condition group, n = 5.

Techniques: In Vivo, Sampling, Expressing

The in vivo assays for the effect of p75NTR knockout on the mRNA expression in dental germs at PN7d. As expected, p75NTR mRNA in the knockout mice was significantly lower than in THE wild-type mice ( p < 0.05). Mage-D1 , Bmal1 , ALP , Col1 , Msx1 , Dmp1 , and Dspp showed the same change in the p75NTR knockout mice, indicating a positive relationship with p75NTR . In contrast, Runx2 showed the reverse change, presenting a negative relationship with p75NTR . Clock , Per1 , Per2 , and Dlx1 showed no significant difference between p75NTR knockout and wild-type mice ( p > 0.05). All experiments were repeated at least three times independently. Data are expressed as mean ± S.D. L. D. Condition group, n = 6; D. D. Condition group, n = 6.

Journal: Frontiers in Physiology

Article Title: A potential role of p75NTR in the regulation of circadian rhythm and incremental growth lines during tooth development

doi: 10.3389/fphys.2022.981311

Figure Lengend Snippet: The in vivo assays for the effect of p75NTR knockout on the mRNA expression in dental germs at PN7d. As expected, p75NTR mRNA in the knockout mice was significantly lower than in THE wild-type mice ( p < 0.05). Mage-D1 , Bmal1 , ALP , Col1 , Msx1 , Dmp1 , and Dspp showed the same change in the p75NTR knockout mice, indicating a positive relationship with p75NTR . In contrast, Runx2 showed the reverse change, presenting a negative relationship with p75NTR . Clock , Per1 , Per2 , and Dlx1 showed no significant difference between p75NTR knockout and wild-type mice ( p > 0.05). All experiments were repeated at least three times independently. Data are expressed as mean ± S.D. L. D. Condition group, n = 6; D. D. Condition group, n = 6.

Techniques: In Vivo, Knock-Out, Expressing

The in vitro assays for the effect of p75NTR over-expression on the mRNA expression in immortalizing stem cells from dental apical papilla (iSCAP). p75NTR was expected to be significantly higher expressed in the over-expression group than that in the control group ( p < 0.05). Mage-D1 , Bmal1 , Clock , Runx2 , Col1 , and Msx1 showed an apparent positive relationship when p75NTR was significantly up-regulated. In contrast, ALP , Dmp1 , and Dspp showed a negative relationship with p75NTR . Per1 , Per2 , ALP , Dlx1 , and Dmp1 showed no significant difference between the over-expression group and control group ( p > 0.05) with lower than that in wild-type mice ( p < 0.05). All experiments were repeated at least three times independently. Data are expressed as mean ± S.D. Overexpression negative control group, n = 3; p75NTR over-expression group, n = 3.

Journal: Frontiers in Physiology

Article Title: A potential role of p75NTR in the regulation of circadian rhythm and incremental growth lines during tooth development

doi: 10.3389/fphys.2022.981311

Figure Lengend Snippet: The in vitro assays for the effect of p75NTR over-expression on the mRNA expression in immortalizing stem cells from dental apical papilla (iSCAP). p75NTR was expected to be significantly higher expressed in the over-expression group than that in the control group ( p < 0.05). Mage-D1 , Bmal1 , Clock , Runx2 , Col1 , and Msx1 showed an apparent positive relationship when p75NTR was significantly up-regulated. In contrast, ALP , Dmp1 , and Dspp showed a negative relationship with p75NTR . Per1 , Per2 , ALP , Dlx1 , and Dmp1 showed no significant difference between the over-expression group and control group ( p > 0.05) with lower than that in wild-type mice ( p < 0.05). All experiments were repeated at least three times independently. Data are expressed as mean ± S.D. Overexpression negative control group, n = 3; p75NTR over-expression group, n = 3.

Techniques: In Vitro, Over Expression, Expressing, Negative Control

Journal: PeerJ

Article Title: Bioinformatical analysis identifies PDLIM3 as a potential biomarker associated with immune infiltration in patients with endometriosis

doi: 10.7717/peerj.13218

Figure Lengend Snippet: The statistical metrics for key differentially expressed genes (DEGs).

Article Snippet: The primary antibodies rabbit anti-PDLIM3 antibody (1:2,000; Servicebio) was used and rabbit anti-β-Actin (1:5,000; ABclonal) served as the internal control.

Techniques:

Journal: PeerJ

Article Title: Bioinformatical analysis identifies PDLIM3 as a potential biomarker associated with immune infiltration in patients with endometriosis

doi: 10.7717/peerj.13218

Figure Lengend Snippet: The enriched GO terms of differentially expressed genes.

Article Snippet: The primary antibodies rabbit anti-PDLIM3 antibody (1:2,000; Servicebio) was used and rabbit anti-β-Actin (1:5,000; ABclonal) served as the internal control.

Techniques: Activation Assay, Clinical Proteomics, Membrane, Binding Assay, Activity Assay

Journal: PeerJ

Article Title: Bioinformatical analysis identifies PDLIM3 as a potential biomarker associated with immune infiltration in patients with endometriosis

doi: 10.7717/peerj.13218

Figure Lengend Snippet: (A) A PPI network of DEGs. (B) A plot of biomarkers selection by LASSO regression algorithm. (C) The receiver operating characteristic (ROC) curve of FMO1, PDLIM3, FAM129A, CLDN11, C3, TMPRSS4 and DEFB1 in the metadata cohort. (D) ROC curve of FMO1, PDLIM3, FAM129A, CLDN11, C3, TMPRSS4 and DEFB1 in the GSE120103 dataset.

Article Snippet: The primary antibodies rabbit anti-PDLIM3 antibody (1:2,000; Servicebio) was used and rabbit anti-β-Actin (1:5,000; ABclonal) served as the internal control.

Techniques: Selection

Journal: PeerJ

Article Title: Bioinformatical analysis identifies PDLIM3 as a potential biomarker associated with immune infiltration in patients with endometriosis

doi: 10.7717/peerj.13218

Figure Lengend Snippet: (A) The expression level of FMO1, PDLIM3, FAM129A, CLDN11, C3, TMPRSS4 and DEFB1 in the GSE120103 dataset. (B) The mRNA expression level of PDLIM3 in endometriosis and control samples. (C) The protein expression level of PDLIM3 in endometriosis and control samples. Mean ± SD, *** P < 0.001.

Article Snippet: The primary antibodies rabbit anti-PDLIM3 antibody (1:2,000; Servicebio) was used and rabbit anti-β-Actin (1:5,000; ABclonal) served as the internal control.

Techniques: Expressing, Control

Journal: PeerJ

Article Title: Bioinformatical analysis identifies PDLIM3 as a potential biomarker associated with immune infiltration in patients with endometriosis

doi: 10.7717/peerj.13218

Figure Lengend Snippet: (A) Correlation between PDLIM3 and single type of immune cell. (B) A comprehensive correlation analysis of PDLIM3 and infiltrating immune cells in endometriosis.

Article Snippet: The primary antibodies rabbit anti-PDLIM3 antibody (1:2,000; Servicebio) was used and rabbit anti-β-Actin (1:5,000; ABclonal) served as the internal control.

Techniques:

Expression of CPXM2 and Pdlim3 in rat cardiomyocytes. H9C2 cells were fixed and double-labelled for confocal indirect immunofluorescence microscopy with the polyclonal ( A ) anti-CPXM2 and ( B ) anti-Pdlim3 antibodies. In the overlay image ( C ) intracellular co-localisation of CPXM2 and Pdlim3 in rat cardiomyocytes is depicted. Nuclear DNA was stained with DAPI (shown in blue). In ( D ) the CPXM2 protein expression in H9C2 as well as a positive control (human recombinant CPXM2) and negative control (empty well) are shown. Bar = 100 μm.

Journal: International Journal of Molecular Sciences

Article Title: MiRNA-29b and miRNA-497 Modulate the Expression of Carboxypeptidase X Member 2, a Candidate Gene Associated with Left Ventricular Hypertrophy

doi: 10.3390/ijms23042263

Figure Lengend Snippet: Expression of CPXM2 and Pdlim3 in rat cardiomyocytes. H9C2 cells were fixed and double-labelled for confocal indirect immunofluorescence microscopy with the polyclonal ( A ) anti-CPXM2 and ( B ) anti-Pdlim3 antibodies. In the overlay image ( C ) intracellular co-localisation of CPXM2 and Pdlim3 in rat cardiomyocytes is depicted. Nuclear DNA was stained with DAPI (shown in blue). In ( D ) the CPXM2 protein expression in H9C2 as well as a positive control (human recombinant CPXM2) and negative control (empty well) are shown. Bar = 100 μm.

Article Snippet: Next, after two washings steps in 1× PBS, samples were incubated with polyclonal ALEXA FLUOR ® 594 conjugated rabbit anti-rat Pdlim3 antibodies (#bs-2928R-A594, Bioss, Woburn, MA, USA) in a 1:200 dilution for 1 h at room temperature in the dark.

Techniques: Expressing, Immunofluorescence, Microscopy, Staining, Positive Control, Recombinant, Negative Control

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